Ilution of anti-rabbit Alexa-Fluor 488 (Invitrogen). Alternatively, ovaries were stained with 1:1,000 dilution
Ilution of anti-rabbit Alexa-Fluor 488 (Invitrogen). Alternatively, ovaries had been stained with 1:1,000 dilution of Nile-Red (10 mg/ml in DMSO, SigmaAldrich). Tissues have been then mounted in DAPI-containing Vectashield medium…